Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms.
In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm.
Recently human and mouse genes encoding a basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported.
The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively.
Homologues of mPer1 designated Per 2 (1257aa) and Per3 (1113aa) have also been cloned.
Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes.
It has been suggested that mPer regulates neuronal activity in the SCN.
Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice.
Clock has been mapped to chromosome 5.
Mouse Clock encodes a transcription factor, a single polypeptide chain of 855aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846aa).
Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney).
Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA:1:10,000-1:100,000 using 50-100ng/well of control peptide
Western Blot: 1:10ug/mlOptimal dilutions to be determined by the researcher.
Control Peptide: P3342-10B
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human Period Clock Protein 1 (RIGUI). Shows no significant sequence homology with Per 2, Per 3, dPer or other known proteins. Species crossreactivity:Species sequence homology: mouse and rat-100%, chicken-65%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide sequence 20aa within the C-terminus of human Per1 (KLH).
