Several peptides associated with feeding behavior have been reported recently.
Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1).
Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis (1,2).
These neuropeptides bind and activate two closely related Orexin receptors—G protein coupled receptors (GPCRs) OX1R and OX2R.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique ELISA: 1:10,000-100,000 Immunohistochemistry: Not tested.
It is recommended that the antibody be tried at 2-20ug/ml.
Other applications not tested.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes mouse Orexin-B. The epitope is not found in Orexin-A. Since Orexins-A and Orexin-B are derived from an unprocessed pre-pro orexins (130aa), the anti-Orexin-B antibodies may also react with pre-pro orexins. Species Crossreactivity: canine, mouse, porcine, rat and human.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：A 13aa peptide mapping near the N-terminus of mouse Orexin-B. The immunogen peptide has 92% homology with rat and human Orexin-B.
