The ion channels activated by glutamate are divided into two classes.
Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).
NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death.
NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Not recommended for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200 for labeled protein A, 1:1000-1:5000 for ECL (NMDAR2A is present in high concentrations in the hippocampus), Immunoprecipitation: Using solubilized hippocampal slices.
3ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200ug rat brain.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid. No preservative added.
Specificity：Recognizes rat NMDAR2A. By Western blot it recognizes a 180kD band in rat brain membranes. Species Crossreactivity: Human, mouse and fish.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：C-Terminal Fusion Protein of rat NMDAR2A (30kD), aa1253-1391.
