The ion channels activated by glutamate are divided into two classes.
Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).
NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death.
NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2,000 using ECL Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:25ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a lyophilized powder from ammonium bicarbonate (5mM) so some residual salt may also be present. Reconstitute with 50ul of PBS. No preservative added.
Specificity:Recognizes the rat NMDAR1 C1 splice variant. By Western blot recognizes a protein with MW of 100kD.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Rt
Immunogen:Peptide corresponding to the C-terminus of the rat NMDAR1 C1 splice variant.