Applications:Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:200-1:1000Immunocytochemistry: 1:200-1:1000Western Blot (ECL): 1:200-1:1000Optimal dilutions to be determined by the researcher.
Immunohistochemistry Protocol:1.
Dissect tissues and freeze on dry ice 2.
Cut on a cryostat -10um slices 3.
Dry slides at RT for 30-90 minutes4.
Fix with cold Acetone/Methanol (50/50) 2 minutes - then either process further or store at -20°C .
Alternative is fixation with 4% PFA and treatment with a trypsin solution.
(0.05% trypsin, incubation 10 minutes at 37°C, followed by 3 washes with PBS5.
Let air dry 6.
PBS 5 minutes 7.
Incubate in 50mM ammonium chloride 30 minutes 8.
PBS 5 minutes 9.
Blocking serum 30-45 minutes 10.
N2851 90 minutes - dilution 1:200 - 1:1000 11.
Wash 3 times for 5 minutes 12.
Secondary fluorescent conjug.
antibody 30 minutes 13.
Wash 3 times for 5 minutes and mount
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes human Nkx5.1, (NK-2 family), a homeodomain transcription factor. MW: 49.152kD Species Crossreactivity: rodent
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Rd
Immunogen：Synthetic peptide corresponding to aa311-328 of human Nkx5.1
