Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+K+Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1) and the K+Cl- cotransporters (KCC1-4).
These co-transporters share a common predicted membrane topology, with 12 TM domains (~500aa), and long hydrophilic, intracellular N- and C-termini containing regulatory phosphorylation sites.
NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells.
The transport process is characterized by electroneutrality and inhibition by the loop diuretic bumetanide, benzametanide and furosemide.
Unlike NKCC1, NKCC2 (human 1099aa, rat/mouse 1095aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney and macula densa.
NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg.
Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting and volume depletion.
Applications:Suitable for use in Western Blot, Immunohistochemistryand ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
Immunohistochemistry (Paraformaldehyde fixed tissue): 2-20ug/mlELISA: 0.5-1ug/ml using 1ug/ml N2801A control peptide per well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes rat sodium, potassium, chloride cotransporter 2 (NKCC2, BSC1, CCC2) at ~150kD. The immunogen shows no significant sequence homology with NKCC1 or TSC.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to 15aa near the C-terminus, cytoplasmic domain of rat NKCC2, comjuagted to KLH. Species Sequence Homology: mouse; 100%; human and rabbit; 87%.
