Chloride is a critical component of all living cells.
It is also the single most dominant diffusible anion inside of most cells - the others are mostly impermeable organic anions.
The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) co- transporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1) and the K+Cl- cotransporters (KCC1-4).
These co-transporters share a common predicted membrane topology, with 12 TM domains (~500aa), and long hydrophilic, intracellular N- and C-termini containing regulatory phosphorylation sites.
NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells.
The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.
NKCC1/BSC2 (human 1212aa, rat 1203aa, and mouse 1205 aa; calculated mol.
wt of ~115kD; actual size is ~145kD) is strongly expressed in the kidney and many other tissues.
In mouse NKCC1 is detected in basolateral membrane of IMCD and in mesangial cells in the glomerulus and basolateral membrane of acinar cells in rat submandubular gland.
In the brain, NKCC1 mRNA is most prominent in choroid plexus.
Applications:Suitable for use in Western Blot and ELISA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes rat sodium, potassium, chloride cotransporter 1 (NKCC1, BSC2, CCC1). No significant sequence homology with other CCC.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corrsponding to 22aa near the C-terminus, cytoplasmic domain of rat NKCC1, conjugated to KLH. Species Sequence Homology: mouse; 95%; human; 86%; bovine; 82%
