Applications:Suitable for use in Immunohistochemistry, Western Blot, ELISA, Inhibition of biological activity in vitro, and in vivo animal studies.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:500-1:5000 Western Blot: 1:500-1:5000ELISA: 1:500-1:5000.
Inhibition of biological activity in vitro: 1:10-1:50.
In vivo animal studies: neatOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:250ul
Host:rabbit
Source Antibody:mouse
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 250ul sterile ddH2O.
Specificity:Recognizes mouse Nerve growth factor-beta (NGF). Less than 1% crossreactivity against recombinant human NT3, NT4 and BDNF by ELISA. Species Crossreactivity: rat, chicken and human NGF-beta. Does not react with bovine NGF.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Ch Hu Mo Rt
Immunogen:Mouse nerve growth factor-beta purified from salivary glands.