84-1030-69　Neurofilament M, 145kD, CT (NF-M) 50ul　N2165-10

特徴

• Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm.
• They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules.
• Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins.
• The neurofilament triplet proteins (68/70, 160, and 200 kD) occur in both the central and peripheral nervous system and are usually neuron specific.
• The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble.
• Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments.
• Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas.
• Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, detected Neurofilament M on 10g of mouse brain lysates
• Immunohistochemistry: 1:200, staining is not affected by neurofilament phosphorylation.
• Optimal Staining With TE Buffer, pH 9.0.
• Epitope Retrieval: Human CerebellumOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.