Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm.
They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules.
Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins.
The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific.
The 68/70kD NF-L protein can self-assemble into a filamentous structure.
The 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble.
Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments.
Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas.
Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung express neurofilaments.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:200Optimal dilutions to be determined by researcher.
Positive Control:Brain, Peripheral Nerve
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat Serum
Form：Supplied as a liquid. No preservative added.
Specificity：Recognizes rat NF-H. Strong and specific staining for NF-H with no crossreactivity for NF-M or NF-L. Species Crossreactivity: human, mouse, bovine and porcine. Does not react with avian NF-H
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Recombinant fusion protein corresponding to the extreme C-terminus of rat NF-H, prepared from E. coli inclusion bodies by dissolution in urea followed by DEAE-cellulose ion exchange chromatography. Does not include the lysine-serine-proline multiphosphorylation repeat and stains neurofilaments irrespective of the level of phosphorylation
