Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot (ECL): 1-10ug/ml.
Calculated MW: ~135kD.
Reported MW for full length NogoA: ~180kD
ELISA: 1:10,000-1:100,000.
Using 50-100ng control peptide per well
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 0.05% sodium azide.
Specificity：Recognizes human Neurite Outgrowth Inhibitor Protein ABC (NogoABC). The immunogen peptide is within the 25 amino acid region identified for the inhibition of neurite outgrowth. No significant sequence homology with other known proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide consisting of 13aa between the putative 2nd-3rd transmembrane domain of human NogoA (KLH coupled). Species Sequence Homology: rat: 92% Nogo or rat foocen-m1.2 protein.
