84-1030-64　［取扱停止］NHE3 (Na, H Exchanger 3) 50ug　N2017

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of Na+ and H+ ions in various tissues.
• The NHE catalyzes the electoneuronal transport of extracellular Na+ for intracellular H+.
• They play a major role in regulation of intracellular pH (pHi) addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders.
• Five isoforms (NHE3-5) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10–12 transmembrane domains.
• The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
• NHE3 is involved in trans-epithelial Na+ absorption.
• The NHE3 mRNA is found in kidney cortex, medulla, jejunum, ileum, colon and stomach (1).
• Applications:Suitable for use ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000 Using 50-100ng control peptide/well
• Western Blot: 1-10ug/ml (ECL).
• A band of ~90kD has been detected in brush border membrane
• Optimal dilutions to be determined by the researcher.
• Control Peptide:N2017-31: NHE3, Rat (Na, H Exchanger 3)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.