84-1030-63　NHE1 (Na, H Exchanger 1, Slc9a1) 50ug　N2015-03

特徴

• Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of Na+ and H+ ions in various tissues.
• The NHE catalyzes the electoneuronal transport of extracellular Na+ for intracellular H+.
• They play a major role in the regulation of intracellular pH (pHi), addition to trans-cellular absorption of Na+, cell volume regulation, and possibly cell proliferation.
• These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, including hypertension, organ growth and hypertrophy, regression of cancer, and renal intestinal disorders.
• Five isoforms (NHE1–5) have been cloned so far.
• They are all similar in their primary structure and predicted to have 10–12 transmembrane domains.
• The COOH-terminals of NHE1, NHE2 and NHE3 are intracellular.
• NHE1 is “House Keeper” and is expressed ubiquitously in all tissues.
• It is implicated in pH homeostasis, volume regulation, and probably cell proliferation.
• It is expressed on the basolateral surface of several epithelia (1).
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1-10ug/ml (ECL).
• ELISA: 1:100,000.
• Using 50-100ng control peptide per well.
• Optimal dilutions to be determined by the researcher.
• Control Peptide:N2015-05: NHE1, Control Peptide, Rat (Na, H Exchanger 1)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.