Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich.
Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath.
MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles (3).
MBP serves as a marker for oligodendrocytes and Schwann cells (3).
MBP is also a marker for malignant schwannomas (4) and appears to play a significant role in the etiology of multiple sclerosis (5–7) and experimental autoimmune encephalomyelitis (8).
Applications:Suitable for use in Immunohistochemistry.
Immunostains myelin basic protein of abnormal appearing oligodendrocytic processes and cell bodies in demyelinating areas.
Recognizes only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerve.
It also stains discrete white matter in brain of multiple system atrophy.
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Grade：Serum
Purity：Serum.
Form：Supplied as a lyophilized powder. Reconstitute with 50ul of sterile ddH2O. Preservative free.
Specificity：Recognizes Myelin Basic Protein in demyelinated nerve tissues. High specificity for lesioned rat spinal cord. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to amino acids 69-86 of the guinea pig Myelin Basic Protein.
