84-1030-55　Monocarboxylate Transporter 4 (MCT4, MOT4) 100ug　M4470-30

特徴

• Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism.
• Lactic acid is produced as the end-product of glycolysis.
• Some tissues, such as white skeletal muscle and red blood cells use this pathway to generate most of their ATP under normal physiological conditions.
• All tissues become dependent on this pathway during hypoxia and ischaemia.
• Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain/maintain a high rate of glycolysis.
• Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis.
• Some tissues such as brain, heart and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells.
• Lactic acid transport is mediated by a group of proton-linked monocarboxylate transporters (MCTs) located at the plasma membrane.
• At least 9 MCTs (MCT1-9)-related proteins have been identified in mammals that are expressed in a tissue specific manner.
• MCT4/MOT4 is most closely related to MCT3.
• It is prominently expressed in skeletal muscle and other cells with a high glycolytic rate such as tumor cells and white blood cells, suggesting an important role in lactic acid efflux.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:10,000-1:100,000 using 50-100ng of M4470-30F control peptide/well.
• Western Blot: 1-5ug/ml using ECL
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.