Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism.
Lactic acid is produced as the end product of glycolysis.
Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions.
All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia.
Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis.
Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis.
Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells.
Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs).
At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner.
MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 494aa, human 500aa; ~95% identity) is a membrane protein containing 12 transmembrane proteins.
MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%).
MCT1 has very wide tissue distribution.
MCT1/MOT1 is ubiquitously expressed but is especially prominent in heart and red muscle.
It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation.
It is the major isoform in tumor cell and erythrocytes.
Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested
Recommended Dilutions:Western Blot: 1-5ug/ml using ECL
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide (Cat #M4470-03E )/well Immunohistochemistry: 3-10ug/ml.
neutral buffered formaldehyde fixed tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity：Recognizes human Monocarboxylate Transporter 1 (MCT1).
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 19aa near the C-terminus, cytoplasmic domain of human MCT1, conjugated to KLH. Species Sequence Homology: hamster; 63%, mouse; 57%
