Metabotropic glutamate receptors have been classified into three groups.
In general, class I receptors (mGluR1 and mGluR5) mediate the excitation of neurons, whereas activation of class II (mGluR2 and mGluR3) and class III (mGluR4a, mGluR7a, mGluR7b and mGluR8) results in a depression of synaptic transmission.
Class I mGluRs are localized in the postsynaptic membrane and are positively coupled to phospholipase C (PLC).
Class II and class III mGluRs localized to the presynaptic membrane and are negatively coupled to adenylate cyclase.
It has been speculated that mGluR2 and/or mGluR3 may play a role in the modulation of the mossy terminal function.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 0.25-1ug/ml detects a single band at ~140kD
Immunocytochemistry: 0.5-2ug/ml using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy.
Cryostat and vibratome sections can be used with or without Triton X-100 treatment
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes rat Metabotropic glutamate receptor 1 alpha (mGluR1a).
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to rat mGluR1at the C-terminal, PNVTYASVILRDYKQSSSTL, conjugated to KLH with glutaraldehyde
