Mannosidase II is a 135kD protein located on the luminal side of golgi membranes.
The antibody is useful as a marker for golgi apparatus and for studing the fate of the golgi complex in diving cells.
Applications:Suitable for use in Immunocytochemistry and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunocytochemistry: 1:100-1:400 on HeLa and COS7 cells fixed with ice-cold methanol.
Formaldehyde fixation can reduce the staining intensity if left in contact with the cells for more than 15-20 minutes
Immunohistochemistry: Cold methanol fixed tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.02% sodium azide.
Specificity：Recognizes human Mannosidase II. Useful marker for Golgi apparatus identification. Species Crossreactivity: Monkey. Does not crossreact with mouse.
Isotype：IgG
Calc Applications Abbrev：IC IHC
Calc Crossreactivity：Hu Mk
Immunogen：Purified peptide from human mannosidase II.
