LDL constitutes 50% of the total lipoprotein mass in plasma and is the major carrier of cholesterol and cholesteryl esters.
LDL levels strongly correlate with coronary heart disease.
Cholesterol is an important normal constituent of the body.
It is part of the structure of cell membranes, bile acids and steroid hormones.
Since cholesterol is water insoluble, most cholesterol is carried in the blood by lipoproteins.
Lipoproteins are large protein-like molecules, including chylomicrons, VLDL, LDL and HDL.
Chylomicrons are lipoproteins that are present shortly after a meal.
They disappear within about 2 hours in "normal" people.
The main function of LDL seems to be to carry cholesterol to various tissues throughout the body.
LDL is sometimes referred to as "bad" cholesterol because elevated levels of LDL correlate most directly with coronary heart disease.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:50-1:500ELISA: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes fully oxidized modifications of human LDL including Cu2+-oxidized LDL, MDA-LDL, HOCL-LDL, but not to other oxidized proteins like MDA-HSA, HOCL-HSA, MDA-HDL, HOCL-HDL (below detection limit). The reaction of native LDL was weak, but clearly detectable (approx. 20%). Minimally oxidized LDL gave a strong binding signal (> 80%).
