The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators and proto-oncogenes products.
LIM domain mediates protein-protein interactions and, with LIM-homeodomain proteins, act as negative regulators of the transcriptional activation function of the protein.
This transcription factor is required for embryonic survival and for the determination of many cell types.
Applications:Suitable for use in Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation and Immunofluorescence.
Western Blot: 1:3000-1:6000 Immunocytochemistry: 1:500 on P19 cell line Immunoprecipitation: 1:200 Immunofluorescence: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：frog
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 0.02M PBS, pH 7.6, 0.09% sodium azide.
Specificity：Recognizes frog Lim-1. Does not crossreact with Lim-5 (the closest homolog of Lim-1 in frog) on tissue sections but does crossreact with Lim-5 in Western Blot and Immunoprecipitation. Species Crossreactivity: human, rat, mouse and fish. Also reacts with Lim-1 from whole-mount fish embryo.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC IP WB
Calc Crossreactivity：Fr Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-terminal portion of frog Lim-1.
