Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion.
Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface.
Both of the subunits contribute to the binding of the ligand.
The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit.
Applications:Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:ELISA/RIA: 1:1000Western Blot: 1:500 Detects Intergrin alpha 3 on 10ug of A431 lysates
Immunoprecipitation: 5ul precipitates alpha3/beta1 from 5x10e6 cells
Immunohistochemistry/Immunofluorescence: 1:500; Acetone-fixed tissue sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human Integrin alpha3 at ~130kD. Specificity determined by immunoprecipitation from a detergent extract of 35S-methionine labeled neuroblastoma cells. No crossreactivity to alpha1, alpha2, alpha4, alpha5, alpha6 or alphaV integrins. Species Crossreactivity: mouse, rat, bovine, equine, sheep, porcine, canine, chicken, goat and hamster.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP RIA WB
Calc Crossreactivity：Bo Ca Ch Eq Gt Hm Hu Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to the C-terminal sequence, CRIQPSETERLTDDY, located in the cytoplasmic domain of the human alpha 3 integrin subunit.
