84-1030-21　Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS) 50ul　I0800-12

特徴

• Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM).
• In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial.
• The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase.
• The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence.
• Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline.
• NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations.
• This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.
• Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 detects INOS on 10ug of mouse liver lysatesImmunocytochemistry: 1:50,000 (Cultured cells)Immunohistochemistry (ABC):1:5000 using tissue sections of rat liverOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.