Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 5ug/ml Immunohistochemistry (frozen and formalin/paraffin): 5-10ug/ml.
Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecipitation: 10ug/mg of protein lysate (using Protein A)Optimal dilutions to be determined by the researcher.
Positive Control:MAD109 cells or breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes heat shock protein 90 beta (HSP90b). No reactivity with HSP90a/HSP86. Species Crossreactivity: rat, mouse and human.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa2-13 from the N-terminus of mouse heat shock protein 90 beta. Cellular localization: Nuclear and Cytoplasmic.
