Heme oxygenase-1 was first purified from rat liver and porcine spleen and shown to have a molecular weight of 32kD.
Increased expression of HO-1 can be induced by a large number of structurally unrelated pharmacological and other agents, as well as heat shock and other forms of cellular stress.
Baseline levels of HO-1 expression are high in certain tissues, including spleen and liver, but enriched enzyme preparations are recommended for experimental analysis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (See cat.
#H1830-61)
Immunohistochemistry: 1:50-1:1000 on human airway biopsy, human spleen, human kidney and porcine arteries.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.01M sodium azide.
Specificity：Recognizes human Heat Shock Protein 32 (Heme Oxygenase-1, HO-1). Species Crossreactivity: rat spleen, liver and testis, Madin-Darby bovine (MDBK) kidney cells and mouse RAW 264.7 cells following stimulation with gamma interferon and lipopolysaccharide. Does not react with canine (MDCK) kidney cells.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa12-25 + Cys-NH2, HDLSEALKEATKEVHC-NH2 of human Heat Shock Protein 32.
