GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth.
While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones.
In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus.
Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000.
Detects a single band at ~50kD on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).
Immunohistochemistry (paraffin): 1:2000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990).
Has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).
Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：feline
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.02% sodium azide.
Specificity：Recognizes feline Growth Associated Protein 43 (GAP-43). Species Reactivity: Human, monkey, mouse, rat and bovine.
