Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft.
By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented.
There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2.
mRNA has been identified in brain for each of these proteins.
EAAT2 transports L-glutamate and also L- and D-aspartate.
It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft.
Acts as a symport by cotransporting sodium.
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): 1:1000-1:4000Immunohistochemistry: 1:5000-10,000Western Blot: 1:500 detected GLT-1 on 10ug of mouse brain lysatesOptimal dilution to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide
Specificity：Recognizes rat Glial Glutamate Transporter GLT1 (EAAT2). Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal of rat GLT-1.
