Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Silver-intensified immunogold or fluorescence.
Samples should be fixed with 0.1-2.5% glutaraldehyde for optimum detection.
This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative).
The minimum glutaraldehyde concentration is 0.00%.
Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2000Tests
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 100mM phosphate buffer, 1% sterile goat serum, 0.05% thimerosal
Specificity：Recognizes Glutamate (L-Glutamate). Calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable crossreactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/Lglutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Crossreactivity: D-glutamate, 1;20; free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue), 1:8.
