GABA is a major inhibitory neurotransmitter, the GABAergic transmission being terminated by the rapid Na+/Cl-dependent uptake through GABA transporters.
It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties.
Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1).
There is ~50% homology between various GABA transporter subtypes.
GABA transporters are predicted to contain 12 potential transmembrane domains.
The NH2 and COOH-termini are predicted to be intracellular.
Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% aa identity).
GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues.
It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.
Applications:Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:100 using silver-intensified immunogold or fluorescence.
Samples should be fixed with 0.05-2.5% glutaraldehyde for optimum detection.
This antibody has also been used and found to work with a zero-low glutaraldehyde/high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer/3% sucrose fixative).
The minimum glutaraldehyde concentration is 0%.
Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C.
For long-term storage, aliquot and store at 4°C.
Do not freeze.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in stabilizing buffer (Phosphate buffer with sterile goat serum) containing 0.05% thimerosal.
Specificity：Recognizes GABA (gamma-aminobutyrIc acid). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue crossreactivity (<1:1000) against L-alanine, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.
