Fibrinogen is synthesized in the liver and platelets and plays a major role in the coagulation process.
It consists of 6 polypeptide chains, two each of A alpha-, B beta- and gamma-chains and is a dimeric molecule, each subunit consisting of one of each chain type.
Thrombin cleaves N-terminal peptides from each of the A alpha-and B beta-chains to form fibrin monomer.
The newly exposed N-terminal sites spontaneously link to C-terminal sites of another fibrin molecule to form a fibrin chain.
These are then crosslinked by activated Factor XIII which stabilizes the fibrin clot.
Normal plasma levels of fibrinogen are around 2.6g/L.
Labeling Ratio:Fluorescein:antibody molar ratio: 2.8-3.3 Absorbance ratio (A493/A276): 0.78±0.05
Applications:Suitable for use in Western Blot, Dot Blot, Immunofluorescence (direct or indirect) and Fluorescence microscopy.
Transmitted Light ExcitationDirect Immunofluorescence: 1:5-1:40Indirect Immunofluorescence: 1:10-1:80Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ml
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 20mM Tris, 70mM sodium chloride, pH 8.0, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity：Recognizes human Fibrinogen. Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.
Isotype：IgG
Calc Applications Abbrev：DB IF WB
Calc Crossreactivity：Hu
Immunogen：Purified fibrinogen derived from pooled human plasma.
