Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements.
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released.
Levels of DAT protein expression are altered by chronic drug administration.
The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction.
It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
Applications:Suitable for use in Western Blot, Immunopreciptiation, Immunohistochemistry, and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL.
An antibody raised against a similar peptide has been shown to react with a broad 80kD band in rat striatum (2) and not cerebellum.
Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55kD.
Immunoprecipitation: 2-10ugImmunohistochemistry: 2-10ug/ml on frozen sections of tissues fixed with 4% paraformaldehyde (2).
ELISA: 0.5-1.0ug/ml using 1ug/ml of DAT control peptide per well.
Optimal dilutions to be determined by the researcher.
Positive Control:Rat brain tissue
Control Peptide:D9020-20
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes Dopamine transporter (DAT). Species crossreactivity: Rat and mouse. Weakly reacts with human and canine.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Mo Rt
Immunogen：An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (1). This region is presumably the cytoplasmic domain.
