84-1029-59　Dopamine Receptor, D2L, D2S 50ug　D9016-10

特徴

• The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine.
• The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
• Two isoforms are reported for the D2 Dopamine Receptor, isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:ELISA: 0.5-1.0ug/mlWestern Blot: 1:1000.
• Detected Dopamine Receptor D2 on 10ug of human brain lysates.
• Immunoprecipitation: 1-10ug/ml.
• Use 100-400ug of protein.
• An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms.
• Immunohistochemistry (Paraformaldehyde fixed sections): 2-10ug/ml
• Optimal dilutions to be determined by the researcher.
• Positive Control:Monkey brain tissue, rat brain lysate
• Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.