The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways.
Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis.
Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system.
The disruption of the DBH gene is thought to cause changes in development and behavior in certain models.
Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.
Applications:Suitable for use in RIA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:2000 on rabbit and guinea pig using an ABC detectionOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Serum
Form：Supplied as a lyohilized powder. No preservative added. Reconstitute with 50ul sterile ddH2O.
Specificity：Recognizes bovine Dopamine beta hydroxylase (DBH). Reacts with a single band on Western Blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme. Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IHC RIA WB
Calc Crossreactivity：Bo Hu Rt
Immunogen：Native enzyme from bovine adrenal glands.
