84-1029-45　Cytochrome P450 Enzyme CYP1A2 100ul　C9095-18A

特徴

• Cytochromes P450 enzymes are a group of heme-thiolate monooxygenases.
• In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway.
• It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
• Most active in catalyzing 2-hydroxylation.
• Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation.
• Also acts in the metabolism of aflatoxin B1 and acetaminophen.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:1000-1:4000.
• Detects a band at ~54kD
• Immunohistochemistry: frozen and formaldehyde treated sections.
• Optimal dilutions to be determined by researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.