By immunoblotting the antibody shows a 5-10 fold increase in staining of a 43kD protein after treatment of cells with agents that increase intracellular cAMP.
The antibody also recognizes phosphorylated ATF-1 due to a common epitope.
The antibody has been preabsorbed with unphosphorylated peptide and will prove to be useful for the investigation of CREB phosphorylation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Cross adsorbed serum
Form：Supplied as a liquid, 0.01% sodium azide.
Specificity：Recognizes phosphorylated Serine133 in cAMP response element binding protein. (CREB). Species Crossreactivity: Human, rat, monkey and mouse.
