Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 and COX2.
Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities.
These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin.
COX2 is a 72kD protein having 60% homology to COX1.
It is induced by cytokines and mitogens.
Most likely, it plays a role in inflammatory diseases such as rheumatoid arthritis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using Chemiluminescence
ELISA: 1:10,000-100,000Optimal dilutions to be determined by researcher.
Control Peptide:C7904-81
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.09% sodium azide.
Specificity：Recognizes human Cyclooxygenase 2. Species crossreactivity: mouse, sheep and bovine. Expected to crossreact with rat based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo Sh
Immunogen：Synthetic peptide corresponding to 17aa from human COX2 (KLH coupled).
