Mouse Connexin 40 is a 358aa gap junction protein with a predicted M.W.
of ~40kD.
It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer, 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994).
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
A connexon is composed of a hexamer of connexins.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000 detected Connexin 40 in 10ug of Jurkat lysates
Immunohistochemistry: 1:50 detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections.
Optimal dilutions to be determined by the researcher.
Control Peptide:C7856-01: Connexin 40, Mouse, Control Peptide (CX40, Gap Junction Alpha 5 Protein, CXA-5)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 15mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes mouse Cx40.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide consisting of 19aa within the C-terminal cytoplasmic domain of mouse Cx40. Species Sequence Homology: human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).
