The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues.
Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element.
Collagen VI is a major structural component of microfibrils.
The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains.
The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively.
The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain).
Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:10-1:40 dilution of reconstitute for indirect immunofluorescent staining of frozen tissue sections.
ELISA: 1:15,000 dilution of reconstitute for ELISA on human collagen type VI.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Pooled antisera was cross-absorbed against immobilized human serum proteins and collagen types IV and V. The IgG fraction was then precipitated with ammonium sulfate and subsequently dialyzed.
Form：Supplied as a lyophilized powder in 10mM Na-phosphate, 150mM sodium chloride, pH 7.5, 0.1% mannitol. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes human Collagen Type VI. Crossreactivity: human collagen type I: <0.5%, Human collagen type II: <0.5%, Human collagen type III: <0.5%, Human collagen type IV: <0.5%, Human collagen type V: 10%, Human collagen type VI: 100 %, Human fibronectin: <0.5% Human albumin: <0.5%, Human immunoglobulin <0.5%
