Applications:Suitable for use in Immunohistochemistry, RIA and ELISA.
Not suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1:80.
Frozen mouse skin and liver tissuesImmunohistochemistry: Acetone or methyl-carnoy fixed, paraffin-embedded tissueRIA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservatives added.
Specificity:Recognizes Mouse Collagen Type IV: 100%, Mouse Collagen Types I, II, III: < 0.1%, Mouse Fibronectin: < 0.1%, Mouse Laminin: < 0.01%, Human Collagen Types IV, V: < 0.1%.
Isotype:IgG
Calc Applications Abbrev:E IHC RIA
Calc Crossreactivity:Mo
Immunogen:Collagen type IV extracted and purified from mouse tumor tissues