Applications:Suitable for use in Dot and Slot Blot, ELISA, Immunohistochemistry and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:*Dot and Slot Blot: 1:100-1:500 ELISA: 1:1000-1:4000 *Immunohistochemistry (Frozen): 1:10-1:40 Immunocytochemistry: 1:10-1:20 Optimal dilutions to be determined by the researcher.
*Note: Harsh chemicals or procedures involving heat may denature collagen epitopes.
We recommend avoiding such actions when using this antibody.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in borate buffered saline, pH 8.2. No preservatives added.
Specificity：Recognizes the conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: bovine and mouse
Isotype：IgG
Calc Applications Abbrev：DB E IC IHC
Calc Crossreactivity：Bo Hu Mo
Immunogen：Human and bovine placental collagen type IV
