This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye.
Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type.
In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
There are two transcripts identified for this gene.
Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Indirect micro-immunofluorescent staining of cryostat human tissue sections: 1:10–1:40 ELISA: 1:4000Western Blot: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% mannitol, 0.1% dextran. No preservative added.
Specificity：Recognizes human collagen type II. Crossreactivity: Collagen type I: 5%, Collagen type II: 90%, Collagen type III: 5%, Collagen type IV: <0.5%, Collagen type V: <0.5%, Fibronectin: <0.5%, Albumin: <0.5%, Immunoglobulin: <0.5%
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Collagen type II extracted and purified from human cartilage.
