Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth.
Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity.
Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300kD molecular weight.
In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis.
In skin the fibrils are interlaced and branched.
Applications:Suitable for use in ELISA, Dot and Slot Blots and Immunohistochemistry.
Not suitable for use in Western Blots or paraffin Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:4000Dot/Slot Blot: 1:500-1:1000Indirect Immunohistochemistry (Frozen sections only): 1:20-1:40Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
Specificity：Recognizes human Collagen Type I. Exhibits <10% crossreactivity with collagen types II, III, IV, V and VI. May crossreact with Collagen Type I from other species. Not tested for crossreactivity with other extracellular matrix proteins (e.g. laminin, fibronectin). Has been cross-adsorbed against collagen types II, III, IV, V and VI.
