This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain.
Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon.
Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Applications:Suitable for use in ELISA, Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilutions: ELISA: 1:4000Indirect Immunofluorescence: 1:10-1:40 on frozen sections or cutured cells.
Paraffin not recommended
Western Blot: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by ammonium sulfate precipation and Ion exchange chromatography.
Form：Supplied as a liquid in PBS pH 7.5, 0.1% Mannitol, 0.1% Dextran. No preservatives added.
Specificity：Recognizes human collagen type I. Species Crossreactivity: Rhesus monkey. Reacts with human collagen type II: <2%, human collagen type III: <10%, human collagen type IV and V: <4%, human fibronectin, human albumin and human immunoglobulin: <0.5%.
