Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure.
Type I collagen (95kD) is found in bone, cornea, skin and tendon.
Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.
Applications:Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen rat skin and liver tissues
RIA: 1:200 ELISANot recommended for paraffin tissue sections or Western blots.
Positive Control: Rat hepatic stellate cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid.
Specificity：Recognizes rat Collagen Type I. Less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.
Isotype：IgG
Calc Applications Abbrev：E IHC RIA
Calc Crossreactivity：Rt
Immunogen：Collagen Type I extracted and purified from rat skin.
