Type I collagen is the most common of the collagens in vertebrates.
It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body.
Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations.
The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc.
Type Icollagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.
Applications:Suitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry.
Not recommended for paraffin tissue sections.
Other applications not tested.
Recommended Dilution:Dot and Slot Blot: 1:100-1:500ELISA: 1:50-1:100Immunohistochemistry (Indirect, frozen sections only): 1:10-1:20 Immunocytochemistry (Indirect): 1:10-1:20 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.
Specificity：Recognizes human type I collagen. Crossreactivity (ELISA): <10% collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Isotype：IgG
Calc Applications Abbrev：DB E IC IHC
Calc Crossreactivity：Hu
Immunogen：Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
