84-1029-06　CD56 (NCAM-1, Neural Cell Adhesion Molecule) 50ug　C2410-69A

特徴

• The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure.
• The family contains over a 100 members that include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule).
• Both NCAM and NgCAM are calcium independent adhesion molecules.
• NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development.
• It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development.
• One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections.
• The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA).
• The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
• Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry, ELISA and Functional Blocking.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot (ECL): 1:1000 on 10ug of mouse brain lysate
• Immunohistochemistry (Wide range of fixatives): 1ug/ml will detect chicken NCAM; 2ug/ml will detect mouse NCAM
• ELISA: 0.3ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Neuraminidase-treated mouse brain membrane
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.