Applications:Suitable for use in ELISA, Radioimmunoassay, Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:ELISA/RIA: 1:1000Western Blot (non-reduced): 1:1000.
Blocking should be with 1-3% BSA.
Recognizes a 140kD band under non-reducing conditions
Immunohistochemistry: 1:500Immunoprecipitiation: 5ul to precipitate alpha4 from 5x10e6 cells
Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes human Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No crossreactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. Recognizes an epitope on the cytoplasmic domain of the protein. Epitope is intracellular. Species Crossreactivity: rat, chicken, hamster and mouse
Isotype：IgG
Calc Applications Abbrev：E IHC IP RIA WB
Calc Crossreactivity：Ch Hm Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal sequence of the human alpha 4 integrin subunit. Cellular Localization: Cytoplasmic; Intracelllular.
