The CD44 molecule belongs to a family of cellular adhesion molecule found on a wide range of normal and malignant cells in epithelial, mesothelial and haematopoietic tissues.
CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (HCAM) molecule.
The additional 10 exons (V1 to V10) are only expressed by alternative splicing of the nuclear RNA.
The expression of specific cell adhesion molecule CD44 splice variants has been shown to be associated with metastasis and poor prognosis in certain human malignancies, such as breast cancer.
Applications:Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Imunohistochemistry and Functional Blocking.
Other applications not tested.
Recommended Dilutions:RIA: 1:5000Immunofluorescence: 1:100-1:200Flow Cytometry: 1:500-1:1000ELISA: 1:1000-1:2000Western Blot: 1:5000-1:10,000Immunohistochemistry (Paraffin): 1:150- 1:250Immunoprecipitation: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium phosphate, 150mM sodium chloride, pH 7.5, 0.01% sodium azide
Specificity：Recognizes human CD44v6 (~200-250kD). Species Crossreactivity: rabbit, porcine, mouse, canine and bovine
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC IP RIA WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rb
Immunogen：Synthetic peptide corresponding to human CD44v6.
