CPH is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.
It is a peripheral membrane protein.
The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000.
Carboxypeptidase H precursor migrates at ~53kD while active enzyme migrates at ~48kD
Immunocytochemistry (AtT-20 Cell line): 1:100-1:500 Immunoprecipitation: 1:250-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.02% sodium azide
Specificity：Recognizes Carboxypepdidase H, C-terminus. Species Crossreactivity: human mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of Carboxypeptidase H, conjugated to KLH.
