Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana.
(-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis.
Cannabis affects cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics.
D9-THC is also immunosuppressive and impairs cell-mediated immunity, humoral immunity and cellular defenses against a variety of infectious agents both in vivo and in vitro.
To date, two subtypes of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified.
The first brain-derived endogenous cannabinoids, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain.
AEA has higher affinity for the CB1 than for the CB2.
Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-10 ug/ml using Chemiluminescence
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide:C1075-75A
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50?glycerol and store at -20°C.
Reconstituted product is stable for 24 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide. Reconstitute with 100ul ddH2O or sterile 40-50% glycerol for long-term storage.
Specificity：Recognizes rat Cannabinoid Receptor 2 (CB2). Species Sequence Homology: mouse: 83%; human: 82%. No significant sequence homology exists with CB1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：An 18 amino acid peptide sequence (KLH coupled) near the cytoplasmic, C-terminus of rat CB2.
