Water is a critical component of all living cells.
Interestingly, tissue membranes show a great degree of water permeability.
Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water.
Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin".
Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues.
AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin.
The lens specific AQP0 represents up to 80% of total lens membrane protein.
Defects in MIP26 are cause of autosomal dominant cataract.
The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP.
The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane.
Most recently, adipose specific AQP-adipose (342aa; AQPAP or AQP7-like) has been cloned.
It facilities water and glycerol transport.
AQP families of proteins are predicted to contain six transmembrane domains.
The N- and C-terminus are predicted to be cytoplasmic.
Applications:Suitable for use in ELISA, Western Blot.
Note: Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands
ELISA: 1:50,000-1:100,000.
Control peptide can be used to coat ELISA plates at 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide:A3002-20: Aquaporin, Adipose Specific, Human (AQPAP, AQP7-Like)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes Aquaporin, adipose specific. The peptide sequence is unique to Aquaporin, adipose specific with no significant homology to any other Aquaporins. Species Crossreactivity: Human.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugate 20-amino acid peptide sequence within the N-terminal domain of human Aquaporin, adipose specific. Epitope Location: N-terminal, cytoplasmic domain.
